abc solution (Vector Laboratories)
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Vector Laboratories
abc solution
Abc Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+abc+elite+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13651886-265-5-12
Average 99 stars, based on 12882 article reviews
Abc Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+abc+elite+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13651886-265-5-12
Average 99 stars, based on 12882 article reviews
abc solution - by Bioz Stars,
2026-10
99/100 stars
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Immunohistochemistry:Article Title: Loss of β-catenin in cholangiocytes promotes hepatocyte reprogramming and vascular remodeling during murine cholestasis Article Snippet: Primary antibodies were CD45 (1:100, sc-53665, Santa Cruz Biotechnology, Dallas, TX); CK19 (1:100, Troma-III, Developmental Studies Hybridoma Bank, Iowa City, IA); αSMA (1:250, 19,425, Cell Signaling Technology, Danvers, MA); CD31 (1:100, 77,699, Cell Signaling Technology, Danvers, MA); LYVE1 (1:300, BAF2125, R&D Systems, Minneapolis, MN); laminin (1:600, L9393, Millipore Sigma, Temecula, CA); E-cadherin (1:1000; 610,405, BD Biosciences, San Jose, CA). .. Immunohistochemistry was performed using the Article Title: Loss of β-catenin in cholangiocytes promotes hepatocyte reprogramming and vascular remodeling during murine cholestasis. Article Snippet: Sirius red images were captured under polarized light using Nikon Eclipse Ni-E scope under x200 magnification. .. Immunohistochemistry was performed using the Incubation:Article Title: The activation of SIRT1-Nrf2 axis exerts beneficial effects against rotenone-induced cognitive deficits in mice through inhibition of neuroinflammation and ferroptosis. Article Snippet: .. Sections were then blocked for 2 h in PBS containing 5 % horse serum and 0.3 % Triton X100, and incubated with primary antibodies (e.g., Iba-1, 1:500, Wako; NeuN, 1:1000, Abcam; PSD95, 1:500, Abcam) at 4◦C for 24 h. Staining was carried out using the Article Title: Irisin reduces neurodegeneration and neuroinflammation in the 6-hydroxydopamine rat model of Parkinson's disease. Article Snippet: Slices were incubated overnight with primary antibodies anti-tyrosine- hydroxylase (TH) (MAB5280, Chemicon - 1:1000), anti-GFAP (G3893, Sigma-Aldrich - 1:1000), or anti-Iba1 (ab5076, Abcam - 1:1000). .. The following day, sections were incubated with biotinylated secondary antibody (1:200, 2 h) followed by the avidin-biotin complex ( Staining:Article Title: The activation of SIRT1-Nrf2 axis exerts beneficial effects against rotenone-induced cognitive deficits in mice through inhibition of neuroinflammation and ferroptosis. Article Snippet: .. Sections were then blocked for 2 h in PBS containing 5 % horse serum and 0.3 % Triton X100, and incubated with primary antibodies (e.g., Iba-1, 1:500, Wako; NeuN, 1:1000, Abcam; PSD95, 1:500, Abcam) at 4◦C for 24 h. Staining was carried out using the Article Title: Epithelial SLPI expression in severe inflammatory bowel disease relates to high IL-17 and neutrophil programming Article Snippet: Sections were blocked for 1 hour at room temperature in Tris buffer (10 mM, pH 8.0) containing 5 mM EDTA (pH 8.0), 0.15 M NaCl, 0.25% gelatin, 0.05% Tween 20, and 10% normal human serum (AB serum, Sanquin) for human tissue or 10% normal mouse serum (Thermo Fisher Scientific) for murine tissue, plus 10% serum matching the species in which the secondary antibody was raised ( ). .. Sections were stained with primary antibody in PBS overnight at 4°C followed by biotinylated secondary antibody for 1 hour at room temperature, developed using Avidin-Biotin Assay:Article Title: Irisin reduces neurodegeneration and neuroinflammation in the 6-hydroxydopamine rat model of Parkinson's disease. Article Snippet: Slices were incubated overnight with primary antibodies anti-tyrosine- hydroxylase (TH) (MAB5280, Chemicon - 1:1000), anti-GFAP (G3893, Sigma-Aldrich - 1:1000), or anti-Iba1 (ab5076, Abcam - 1:1000). .. The following day, sections were incubated with biotinylated secondary antibody (1:200, 2 h) followed by the avidin-biotin complex ( other:Article Title: The median preoptic nucleus is a key site for estradiol regulation of sleep-wake behaviors in females rats. Article Snippet: Study Objective s: Women experience more sleep disruptions than men, particularly during hormonal transitions such as puberty, pregnancy, and menopause.. This study investigated the role of estradiol (E2) in regulating sleep–wa ke behavior in female rats and identified the brain re gions involve d. Methods: Using an exogenous E2 replacement model in ovariectomized rats, we assessed changes in sleep–wake patterns via electroencephalographic/electromyogram telemetry.. The effects of E2 and progesterone, selective estrogen receptor agonists, a nd direct brain infusions of E2 and receptor antag onists were e valuated. |